Coding

Part:BBa_K3221111:Design

Designed by: lingfeng mao   Group: iGEM19_SCU-China   (2019-10-01)


cns1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1288
    Illegal BglII site found at 1875
    Illegal BglII site found at 2293
    Illegal BamHI site found at 2377
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 711
    Illegal SpeI site found at 1240
    Illegal SpeI site found at 1894
    Illegal AgeI site found at 1354
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

condon optimizing in saccharomyces cerevisiae

Source

commercial chemical synthesized https://www.sangon.com

References

Xia Y , Luo F , Shang Y , et al. Fungal Cordycepin Biosynthesis Is Coupled with the Production of the Safeguard Molecule Pentostatin[J]. Cell Chemical Biology, 2017:S2451945617303276.