Translational_Unit

Part:BBa_K322007:Design

Designed by: Chris French and Maria Kowal   Group: iGEM10_Edinburgh   (2010-09-26)

Lumazine protein (LumP). Causes blue shift of P. phosphoreum luciferase emmision spectrum.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 354
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a fixed version of BBa_K216007 submitted by Edinburgh 2009. The sequencing results shows that it differs in a number of mutations from the sequence submitted for BBa_K216007. It is also in pSB1C3.

Source

Source: genomic DNA of Photobacterium phosphoreum.

References

O'Kane, D. J. & Lee, J. (1985). Chemical characterization of lumazine protein from Photobacterium leiognathi: comparison with lumazine protein from Photobacterium phosphoreum. Biochemistry 24, 1467-1475.