Project

Part:BBa_K3218002

Designed by: Abraham Grosman   Group: iGEM19_MSP-Maastricht   (2019-10-15)


a 'dead' Cas9; dCas9 is an inactive endonucelase

dCas9 can bind to promoter regions on the DNA altering expression of chosen gene or be used as a targeting device for other endonucleases. In this case, the dCas9 will be fused to an endonuclease and target a specific area of the gene for mutation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3432
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 977
    Illegal BglII site found at 1436
    Illegal BglII site found at 1525
    Illegal BglII site found at 2170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None