RNA

Part:BBa_K3218001

Designed by: Abraham Grosman   Group: iGEM19_MSP-Maastricht   (2019-10-15)


Ribozyme - gRNA -Ribozyme cassette for expression of gRNA using self cleaveage mediated by the riboz

gRNA cassette for expression of gRNA that is not homologues to either DH5alpha E.coli or MAV203 S.cerevisiae genomes. This mechanism is used coupled to the introns created in BBa_K3218000. The gRNA is flanked by a hammerhead ribozyme and a hepatitis delta virus ribozyme both serve to cleave the RNA releasing the functional gRNA .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 360
    Illegal NheI site found at 865
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
    Illegal NgoMIV site found at 82
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 585
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None