DNA

Part:BBa_K3215017:Design

Designed by: Daniel Oppermann Peixoto   Group: iGEM19_UFRGS_Brazil   (2019-10-19)


phnC Promoter homology


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 33
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 33
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 33
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 33
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The recommended recombination method relies on lambda Red recombination


Source

E. coli K-12 genome

References