DNA

Part:BBa_K3215003

Designed by: Daniel Oppermann Peixoto   Group: iGEM19_UFRGS_Brazil   (2019-09-07)


phnDE homology

This part is a 50bp homologous sequence that occurs between the end of phnD and the beginning of phnE cistrons of the phn Operon from E. coli K-12. It can be used together with phnGH homology part to form a flanking region to insert a construct of interest inside the phn Operon through recombination techniques, removing the original phnEFG (e.g. BBa_K3215005).

2019 UFRGS Brazil phnDE.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None