Part:BBa_K3215001
phnE optimized
Since the phnE coding sequence from E. coli K-12 is defective, this part provides the DNA sequence obtained from E. coli B, which has a normal function, optimized for the E. coli K-12 translation machinery.
Usage and Biology
The phn Operon is one of the nine transcriptional units that are present in the Phosphate Regulon (Pho), a global regulatory mechanism involved in bacterial Pi management. It is responsible for the catabolism of phosphates and it has 14 cistrons (phnCDEFGHIJKLMNOP) that, together, are responsible for controlling the Carbon-Phosphorus (C-P) lyase activity. The C-P lyase pathway is activated upon conditions of phosphate starvation and it is responsible for converting phosphonates into 5-phosphoribosyl-α-1-diphosphate (PRPP), utilizing ATP.
phnCDE
It encodes an ATP-binding cassette, where phnC has the ATP-binding site, phnD is a periplasmic binding protein for phosphonates and phnE is a membrane spanning transport protein.
The complex formed is composed of two proteins derived from phnC, two from phnE and one solute-binding protein from phnD. Altogether, these proteins are responsible for the transport of alkylphosphonates and aminoalkylphosphonates, through the reactions:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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