Part:BBa_K3208011:Design

Part BBa_K3208000 modified with red expression cassette

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 291
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1087
  Illegal AgeI site found at 1199
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1336
  Illegal BsaI.rc site found at 301
  

Design Notes

This part was made by PCR amplification of BBa_K3208010 with primers designed in a way to allow its cloning into BBa_K3208000 after Eco31 digestion.

Source

This par is a combination of parts BBa_K3208000 and BBa_K3208010.