Coding

Part:BBa_K3208011

Designed by: Slavil Peykov   Group: iGEM19_Bulgaria   (2019-10-21)


Part BBa_K3208000 modified with red expression cassette

This is a modified version of part BBa_K3208000. This new part contains red expression cassette (BBa_ K3208010) inserted between the two Eco31I sites. This allows selection of positive transformants based on colour when one uses these 2 sites for cloning.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1087
    Illegal AgeI site found at 1199
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1336
    Illegal BsaI.rc site found at 301


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Categories
Parameters
None