Coding

Part:BBa_K3208009

Designed by: Slavil Peykov   Group: iGEM19_Bulgaria   (2019-10-21)


Expression module that produces Carcharin_9

This part is an expression module that produces Carcharin_9. Carcharin_9 is a putative antimicrobial peptide found in the genome of the great white shark.


Charcharin_9: MLELVGNAAQGNKKTCIILRHLQL


The expression module contains:

- T7 promoter (2-20)

- strong RBS (44-66)

- Charcharin_9's CDS (74-145) with a natural ATG codon

- stop codon (146-149)

- T7 terminator (156-203).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 21


[edit]
Categories
Parameters
None