Coding

Part:BBa_K3208001

Designed by: Slavil Peykov   Group: iGEM19_Bulgaria   (2019-10-21)


Expression module that produces Carcharin_1


This part is an expression module that produces Carcharin_1. Carcharin_1 is a putative antimicrobial peptide found in the genome of the great white shark.


Charcharin_1: SRSSRAGLQFPVGRVHRLQRK


The expression module contains:

- T7 promoter (2-20)

- strong RBS (44-66)

- start codon ATG (74-76)

- Charcharin_1's CDS (77-139)

- stop codon (140-142)

- T7 terminator (150-197).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 21


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Categories
Parameters
None