Composite

Part:BBa_K3206014:Design

Designed by: Karen Fung   Group: iGEM19_Newcastle   (2019-10-16)


His-tagged eGFP under Bba_J23100 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 777
    Illegal BamHI site found at 783
    Illegal BamHI site found at 789
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed to be assembled by Type IIs


Source

Derived from Aequeora victoria. All basic parts were derived from the iGEM registry to form this composite part.

References