Coding

Part:BBa_K3206012:Design

Designed by: Karen Fung   Group: iGEM19_Newcastle   (2019-10-16)


His-tagged eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 715
    Illegal BamHI site found at 721
    Illegal BamHI site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to be assembled using Type IIs


Source

Derived from Aequeora victoria. eGFP sequence was derived from BBa_K2680546

References