Composite

Part:BBa_K3206010:Design

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-16)


adhEp2 promoter with RFP fluorescent reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 648
    Illegal AgeI site found at 760
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part is made up of a promoter that should become active in the presence of eicosanol (BBa_K3206011), an RBS (BBa_B0034), RFP CDS (BBa_E1010) and a terminator (BBa_B0015). This part was made compatible with Type IIs assembly. BsaI restriction sites were added and fusion sites were made compatible with a DVK-AF level-1 pOdd destination vector. This part was designed in benchling and ordered as a gBlock from IDT.


Source

All basic parts used to make this composite part were obtained from iGEM's Registry of Standard Biological Parts.

References