Composite

Part:BBa_K3206000:Design

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-14)


Glycine biosensor with gcvBp promoter region and RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 670
    Illegal AgeI site found at 782
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part is made up of a gcvBp promoter (BBa_K3206001), a ribosome binding site (BBa_B0034), CDS region coding for a RFP reporter (BBa_E1010) and a terminator (BBa_B0015 terminator). The composite part was made compatible with Type IIs assembly, with prefix/suffix BsaI restriction sites and fusion sites (5' end-GGAG, 3' end- CGCT) compatible with a level-1 pOdd destination vector, specifically Dvk-AF. Composite part was designed in benchling and ordered as a gBlock from IDT.


Source

Composite part are made of named basic parts found within the iGEM Registry of Standard Parts

References

Blattner FR, Plunkett G, Bloch CA, et al (1997) The Complete Genome Sequence of Escherichia coli K-12. Science (80- ) 277:1453 LP – 1462. doi: 10.1126/science.277.5331.1453

Ghrist A, Heil G, Stauffer G (2001) GcvR interacts with GcvA to inhibit activation of the Escherichia coli glycine cleavage operon. Microbiology 147:2215–2221. doi: 10.1099/00221287-147-8-2215

Mischley LK, Standish LJ, Weiss NS, et al (2016) Glutathione as a Biomarker in Parkinson’s Disease: Associations with Aging and Disease Severity. Oxid Med Cell Longev 2016:. doi: 10.1155/2016/9409363

OKAMURA-IKEDA K, OHMURA Y, FUJIWARA K, MOTOKAWA Y (1993) Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system. Eur J Biochem 216:539–548. doi: 10.1111/j.1432-1033.1993.tb18172.x