Regulatory

Part:BBa_K3205001:Design

Designed by: Shengkun Tong   Group: iGEM19_HZAU-China   (2019-10-07)


Pare promoter region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The binding sequence of AreR regulatory protein is a stem-loop structure that can act as the terminator of upstream genes.


Source

The sequences of this promoter come from Acinetobacter sp. Strain ADP1 genome.

References

1.Jones R M, Williams P A. areCBA Is an Operon in Acinetobacter sp. Strain ADP1 and Is Controlled by AreR, a ς54-Dependent Regulator[J]. Journal of bacteriology, 2001, 183(1): 405-409.

2.Jones R M, Williams P A. Mutational analysis of the critical bases involved in activation of the AreR-regulated σ54-dependent promoter in Acinetobacter sp. strain ADP1[J]. Appl. Environ. Microbiol., 2003, 69(9): 5627-5635.