Regulatory
Part:BBa_K3205001:Design
Designed by: Shengkun Tong Group: iGEM19_HZAU-China (2019-10-07)
Pare promoter region
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The binding sequence of AreR regulatory protein is a stem-loop structure that can act as the terminator of upstream genes.
Source
The sequences of this promoter come from Acinetobacter sp. Strain ADP1 genome.
References
1.Jones R M, Williams P A. areCBA Is an Operon in Acinetobacter sp. Strain ADP1 and Is Controlled by AreR, a ς54-Dependent Regulator[J]. Journal of bacteriology, 2001, 183(1): 405-409.
2.Jones R M, Williams P A. Mutational analysis of the critical bases involved in activation of the AreR-regulated σ54-dependent promoter in Acinetobacter sp. strain ADP1[J]. Appl. Environ. Microbiol., 2003, 69(9): 5627-5635.