Coding

Part:BBa_K3204002:Design

Designed by: Felix Schweigkofler   Group: iGEM19_NAWI_Graz   (2019-10-16)


Receptor binding protein of paenibacillus phage HB10c2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Three internal PstI sites have been removed, without altering the amino acid sequence.


Source

The source of this part is the paenibacillus phage HB10c2. The gene has been codon optimized for the expression in E. coli with the tool from Thermo scientific

References