Composite

Part:BBa_K3202052:Design

Designed by: Heyuan Ni   Group: iGEM19_BHSF_ND   (2019-10-16)


AraC-Pc-pBAD-ParE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1375
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

N/A


Source

Synthesized

References

【1】A RelE/ParE superfamily toxin in Vibrio parahaemolyticus has DNA nicking endonuclease activit Biochemical and Biophysical Research Communications, 15 July 2017, Pages 29-34

【2】Structural and Functional Analysis of the Kid Toxin Protein from E. coli Plasmid R1. Structure, Volume 10, Issue 10, October 2002, Pages 1425-1433