Composite

Part:BBa_K3202051:Design

Designed by: Heyuan Ni   Group: iGEM19_BHSF_ND   (2019-10-16)


AraC-Pc-pBAD-TetR-RBS2-sfGFP-T500-T1/TE-MicCsRNA-sRNA Binding Site-PLtetO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1856
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1879


Design Notes

N/A


Source

Synthesized

References

【1】Aiba H (2007) Mechanism of RNA silencing by Hfq-binding small RNAs. Current Opinion in Microbiology

【2】Anthony LC, Suzuki H, Filutowicz M (2004) Tightly regulated vectors for the cloning and expression of toxic genes. Journal of Microbiological Methods 58: 243-250

【3】Aparicio T, de Lorenzo V, Martínez-García E (2017) Broadening the SEVA Plasmid Repertoire to Facilitate Genomic Editing of Gram-Negative Bacteria. In Hydrocarbon and Lipid Microbiology Protocols: Genetic, Genomic and System Analyses of Pure Cultures

【4】McGenity TJ, Timmis KN, Nogales B (eds), pp 9-27. Berlin, Heidelberg: Springer Berlin Heidelberg Balzer S, Kucharova V, Megerle J, Lale R, Brautaset T, Valla S (2013) A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli. Microbial cell factories

【5】Bervoets I, Charlier D (2019) Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.

【6】Lopez-Walle B, Gauthier M, Chaillet N (2008) Principle of a submerged freeze gripper for micro-assembly. IEEE Trans Robotics 24(4):897–902

【7】Rakotondrabe M, Haddab Y, Lutz P (2009) Development, modelling and control of micro/nano positionning 2 dof stick-slip device, IEEE/ASME Trans. Mechatronics 14(6):733–745

【8】M. Rakotondrabe Y. Haddab and P. Lutz, "Design, development and experiments of a high stroke-precision 2DoF (linear-angular) microsystem" in Proc. 2006 IEEE Int. Conf. on Robotics and Automation: pp. 669 - 674.