Composite
Part:BBa_K3198007:Design
Designed by: Chunyang Song Group: iGEM19_NUS_Singapore (2019-09-27)
HicA-LuxABCDE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 549
Illegal NheI site found at 1767
Illegal NheI site found at 4733 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3731
Illegal BamHI site found at 489
Illegal BamHI site found at 1706
Illegal XhoI site found at 4561 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1541
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6120
Illegal BsaI.rc site found at 3129
Illegal SapI site found at 1523
Illegal SapI.rc site found at 6445
Design Notes
NIL
Source
HicA originated from E. coli K12 and its sequence was synthesized by IDT. LuxABCDE sequence is obtained from iGEM repository.