Composite
Part:BBa_K3196014:Design
Designed by: Hao Qiu Group: iGEM19_HUST-China (2019-10-16)
AOX1-Kozak-PHO1 pro-SLAC-His tag-AOX1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 937
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1568
Design Notes
protein
Source
References
[1] T. Kudanga, M. Le Roes-Hill, Laccase applications in biofuels production: current status and future prospects, Appl. Microbiol. Biotechnol., 98 (2014), pp. 6525-6542;
[2] D.M. Mate, M. Alcalde, Laccase engineering: from rational design to directed evolution, Biotechnol. Adv., 33 (2015), pp. 25-40;
[3]D. Yadav, B. Ranjan, N. Mchunu, M.L. Roes-Hill, T. Kudanga. Secretory expression of recombinant small laccase from Streptomyces coelicolor A3(2) in Pichia pastoris, Int. J. Biol. Macromol., 108 (2018), pp. 642-649;