Coding

Part:BBa_K3195002:Design

Designed by: JingYuan Liu   Group: iGEM19_SEU-Nanjing-China   (2019-08-22)


GST tagged carboxypeptidase Z/N


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
    Illegal XhoI site found at 1939
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1906
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

PLEASE ADD MORE


Source

Found in amphioxus (Branchiostoma)

References

[1]Ahn, S. J., Kim, N. Y., Jeon, S. J., Sung, J. H., Je, J. E., Seo, J. S., … Lee, H. H. (2008). Molecular cloning, tissue distribution and enzymatic characterization of cathepsin X from olive flounder ( Paralichthys olivaceus). Comparative Biochemistry and Physiology, Part B, 151(2), 203–212. https://doi.org:8118/10.1016/j.cbpb.2008.07.001
[2]He Chunpeng, Han Tingyu, Liao Xin, Zhou Yuxin, Wang Xiuqiang, Guan Rui, Tian Tian, Li Yixin, Bi Changwei, Lu Na, He Ziyi, Hu Bing, Zhou Qiang, Hu Yue, Lu Zuhong and Chen J.-Y. Phagocytic intracellular digestion in amphioxus (Branchiostoma)285Proc. R. Soc. B http://doi.org/10.1098/rspb.2018.0438
[3]Reznik, S. & Fricker, L. CMLS, Cell. Mol. Life Sci. (2001) 58: 1790. https://doi.org/10.1007/PL00000819