Part:BBa_K3192022

phaC

This part contains the sequence for phaC, the third gene involved in the biosynthesis of Polyhydroxybutyrate (PHB). The phaC encodes for the Poly(3-hydroxyalkanoate) polymerase subunit PhaC protein, which polymerizes (R)-3-hydroxybutyryl-CoA to create polyhydroxybutyrate (PHB). The product consists of thousands of hydroxybutyrate molecules linked end to end.¹

Structure of Poly(3-hydroxybutyrate) polymerase subunit PhaC enzyme.¹

References:

1) UniProt Consortium European Bioinformatics Institute Protein Information Resource SIB Swiss Institute of Bioinformatics. Poly(3-hydroxyalkanoate) polymerase subunit PhaC. UniProt Consortium European Bioinformatics Institute Protein Information Resource SIB Swiss Institute of Bioinformatics (2019). Available at: https://www.uniprot.org/uniprot/P23608. (Accessed: 21st October 2019) Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 564
  Illegal BamHI site found at 856
  Illegal BamHI site found at 1426
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1063
  
• 1000
  COMPATIBLE WITH RFC[1000]