Coding

Part:BBa_K3192020:Design

Designed by: Simonne Guenette   Group: iGEM19_Virginia   (2019-10-13)


phaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 265
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part DNA sequence was taken from NCBI and codon optimized for E. coli K12 chassis. The sequence was then reviewed for any illegal restriction sites and conservative edits were made as necessary. The coding sequence was then designed for Golden Gate (using Teselagen Software) assembly and then synthesized. This part was assembled into a larger composite part using Golden Gate Assembly and then sequenced for identity confirmation.


Source

Cupriavidus nectar

References

Cupriavidus necator H16 PhaC1 (phaC1), PhaA (phaA), and PhaB1 (phaB1). (n.d.). Retrieved from https://www.ncbi.nlm.nih.gov/nuccore/MH558939.1.