Reporter

Part:BBa_K3190205:Design

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-14)


sfGFP: Superfolder GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

We use the coding sequence of sfGFP to add it to the C-terminus of the coding sequence of the receptor along with a linker. This is done to carry out a co-localisation assay, to determine the protein expression and to confirm the alignment of the receptor molecule.


Source

Aequorea victoria

References