![](https://parts.igem.org/images/partbypart/icon_signalling.png)
Signalling
Part:BBa_K3190203:Design
Designed by: Ojas Tulsyan Group: iGEM19_UCopenhagen (2019-10-14)
STE12: Transcription factor downstream MAPK
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 640
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1413
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 640
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 640
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 850
Illegal BsaI.rc site found at 1563
Design Notes
The STE12 transcription factor is activated downstream of the MAPK cascade when phosphorylated by Fus3p. It then binds to the pheromone response element (PREs) upstream of the target genes, we therefore exploit this mechanism to make the activated STE12 bind to the promoter of our reporter gene i.e. pFIG1
Source
S. cerevisiae