Coding

Part:BBa_K3190104:Design

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-13)


Homo sapiens luteinizing hormone/choriogonadotropin receptor (LHCGR), CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1643
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 1324
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1558


Design Notes

The gene encoding for the receptor was codon optimised and coupled to the strongest constitutive promoter pCCW12 for heterologous expression in S. cerevisiae, wherein we expect that upon binding of LH to the external part of the membrane spanning receptor, a transduction of the signal takes place that activates the G protein that is bound to the receptor internally. With LH attached, the receptor shifts conformation and thus mechanically activates the G protein, which detaches from the receptor and activates the MAPK system.


Source

Homo sapiens

References