Regulatory

Part:BBa_K3190002:Design

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-12)


pCCW12: Yeast strong constitutive promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 298
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 298
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 298
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter is used in our 5 constructs with receptors (GPER, GPER-sfGFP, XLHCGR, XLHCGR-sfGFP, Hu-LHCGR, Hu-LHCGR-sfGFP), which is used for genomic integration of the receptor genes in our modular system.


Source

S. cerevisiae

References