Composite

Part:BBa_K3189013:Design

Designed by: Jehoshua Sharma, Nathanael Wilms, Connor Gianetto-Hill   Group: iGEM19_Guelph   (2019-10-16)


Lac-regulated VioABE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 4066
    Illegal XbaI site found at 1304
    Illegal XbaI site found at 2748
    Illegal XbaI site found at 5932
    Illegal PstI site found at 3607
    Illegal PstI site found at 4647
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 4066
    Illegal NheI site found at 1169
    Illegal NheI site found at 2613
    Illegal NheI site found at 5797
    Illegal NheI site found at 6557
    Illegal PstI site found at 3607
    Illegal PstI site found at 4647
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 4066
    Illegal BamHI site found at 1455
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 4066
    Illegal XbaI site found at 1304
    Illegal XbaI site found at 2748
    Illegal XbaI site found at 5932
    Illegal PstI site found at 3607
    Illegal PstI site found at 4647
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 4066
    Illegal XbaI site found at 1304
    Illegal XbaI site found at 2748
    Illegal XbaI site found at 5932
    Illegal PstI site found at 3607
    Illegal PstI site found at 4647
    Illegal NgoMIV site found at 2525
    Illegal NgoMIV site found at 2906
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Addgene

References