Composite

Part:BBa_K3187012:Design

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)


pTet sfGFP-P22 Bacteriophage Scaffolding Protein x Coat Protein pT7 (Convergent)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
    Illegal XbaI site found at 2900
    Illegal PstI site found at 1334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal NheI site found at 1339
    Illegal PstI site found at 1334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
    Illegal BamHI site found at 796
    Illegal XhoI site found at 1499
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
    Illegal XbaI site found at 2900
    Illegal PstI site found at 1334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
    Illegal XbaI site found at 2900
    Illegal PstI site found at 1334
    Illegal NgoMIV site found at 1185
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 94


Design Notes

Codon-optimized for E. coli


Source

Ordered from Integrated DNA Technologies (IDT)

References