Composite
Part:BBa_K318517:Design
Designed by: Sarah R. Sandock Group: iGEM10_Wisconsin-Madison (2010-09-26)
Pc + RamA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 159
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 159 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 159
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 159
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The ramA gene was PCR amplified from Salmonella enterica strain LT2 and was cloned behind the constitutive promoter J23100 to have RamA present in the cell. In our experiment, we were using RamA to bind to cholic acid (bile salt).
Source
The Salmonella enterica strain LT2 chromosomal DNA came from Diana Downs' Lab at the University of Wisconsin-Madison.
References
Nikaido E., A. Yamaguchi, and K. Nishino. 2008. AcrAB Multidrug Efflux Pump Regulation in Salmonella enterica serovar Typhimurium by RamA in Response to Environmental Signals. J. Biol. Chem. 283:24245-24253.