Coding

Part:BBa_K318516:Design

Designed by: Sarah R. Sandock   Group: iGEM10_Wisconsin-Madison   (2010-09-26)

RamA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 116
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 116
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ramA gene was PCR Amplified from Salmonella enterica strain LT2. The ramA gene make the RamA protein, which binds to cholic acid (bile salts).

Source

The Salmonella enterica strain LT2 chromosomal DNA came from Diana Downs' Lab at the University of Wisconsin-Madison.

References

Nikaido E., A. Yamaguchi, and K. Nishino. 2008. AcrAB Multidrug Efflux Pump Regulation in Salmonella enterica serovar Typhimurium by RamA in Response to Environmental Signals. J. Biol. Chem. 283:24245-24253.