Coding

Part:BBa_K3183202

Designed by: Natasha Cooke   Group: iGEM19_Oxford   (2019-10-16)


pET28A-AgrBD

AgrBD is used by C. difficile to produce its quorum signalling molecule, also known as the auto-inducer peptide (AIP). This part is designed to be used in E. coli to enable purification and characterisation of thie elusive protein, which is linked to toxin synthesis in C. difficile 1. This part also contains a His tag after each protein to allow individual purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.

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Categories
Parameters
None