Protein_Domain
MSG

Part:BBa_K3183025:Design

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-16)


Start Methionine-Glycine-Serine Linker Codon-Optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker is typically assembled onto larger parts during PCR, by including its sequence as an overhang to the appropriate primer. The PCR product can then be further assembled into more complex constructs by Gibson Assembly.


Source

Made by Oxford 2019

References