Coding
CD27L1-179

Part:BBa_K3183012:Design

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-14)


CD27L Endolysin Residues 1-179 Targetting C. difficile Optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization matched to E. coli codon tables (Kazusa Database)whilst matching all existing RF standards and removing repetitive sequences for easy de novo DNA synthesis.

Source

Protein sequence derived from Mayer et al., 20111. Synthesised as an IDT gBlock.

References

Mayer, Melinda J et al. “Structure-based modification of a Clostridium difficile-targeting endolysin affects activity and host range.” Journal of bacteriology vol. 193,19 (2011): 5477-86. doi:10.1128/JB.00439-11