Coding
mClover3

Part:BBa_K3183010:Design

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-14)


mClover3 Fluorescent Protein, Codon Optimized for L. reuteri


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was undertaken to conform to RF10 standards and to match existing codon usage tables for Lactobacillus reuteri.


Source

This part was originally characterized by Bajar et al, 2016 through evolution of GFP derivative Clover2: https://www.nature.com/articles/srep20889

References

1. Bajar, Bryce T., et al. “Improving Brightness and Photostability of Green and Red Fluorescent Proteins for Live Cell Imaging and FRET Reporting.” Scientific Reports, vol. 6, no. 1, Feb. 2016, p. 20889. DOI.org (Crossref), doi:10.1038/srep20889.