Regulatory
AgrProm

Part:BBa_K3183003:Design

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-16)


Putative Promoter Regulated by AgrA2 in C. difficile


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RF10, 12, 21, 23, 25, and 1000 Compatible

Source

Derived from the C. difficile strain CD196 Genome by selecting the upstream region of agr operon; synthesized in IDT gBlocks.

References

Martin, Melissa J., et al. “The Agr Locus Regulates Virulence and Colonization Genes in Clostridium Difficile 027.” Journal of Bacteriology, vol. 195, no. 16, Aug. 2013, pp. 3672–81. PubMed, doi:10.1128/JB.00473-13.