Regulatory

Part:BBa_K3179002

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-13)


miR 885-5p-target

MiRNA 885-5p-target is constructed bearing four repeats of miRNA 885-5p, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.
This part, four repeating sequences complementary to miRNA885-5p on an mRNA, is a 4X miRNA target designed to target miRNA885-5p. When miRNA885-5p reaches a certain concentration in the cell, it would combine with miR885-5p-target and suppress the translation of mRNA. According to our design, miR885-5p-target is designed to regulate the translation of mRNA with response parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None