Coding

Part:BBa_K317003

Designed by: Yoshiyuki MURAKAMI   Group: iGEM10_Tokyo-NoKoGen   (2010-10-19)

NpSRII-9a.a.linker_NpHtrII-StTar

This fusion chimera mediate retinal-dependent phototaxis responses by Escherichia coli, establishing that the nine-helix membrane portion of the receptor-transducer complex is a modular functional unit able to signal in heterologous membranes.

We confirmed that phototaxis device (BBa_K317028) properly works as we expected. This result shows that this part will be work with the combination of the series of promotors and ribosome binding sites.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1789
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 331
    Illegal AgeI site found at 1591
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1027
    Illegal BsaI.rc site found at 1306
    Illegal SapI site found at 801
    Illegal SapI.rc site found at 1807


[edit]
Categories
Parameters
n/aNpSRII-9a.a.linker_NpHtrII-StTar