Composite

Part:BBa_K3165057:Design

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-20)


T7 - nMag + T7 - pMag + mOrange (for Bacillus subtilis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 828
    Illegal BsaI site found at 1723
    Illegal BsaI.rc site found at 2257
    Illegal BsaI.rc site found at 2296


Design Notes

This part has been codon optimised for Bacillus subtilis.


Source

Assembled the individual Biobrick components.

References