Composite

Part:BBa_K3165056:Design

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-20)


N-T7 - nMag + C-T7 - pMag + Gp2 (for Escherichia coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2367
    Illegal NheI site found at 2390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1678
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3800
    Illegal AgeI site found at 901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1350
    Illegal BsaI.rc site found at 2874


Design Notes

This part has been codon optimised For Escherichia coli.

Source

Assembled from individual BioBrick components.

References