Coding

Part:BBa_K3165011:Design

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-18)

C-Terminal T7 RNAP Domain + pMag (Optimised for Escherichia coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1371
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 445


Design Notes

To generate the two separate domains of the T7 RNA Polymerase, the protein was cleaved at the 563rd - 564th amino acid and linked to the light-sensitive heterodimerizing units via GGSGG linker sequences. This part was codon optimised for Escherichia coli.


Source

The sequence of this part was derived from the supplementary material of "Dynamic blue light-inducible T7 RNA polymerases (Opto-T7RNAPs) for precise spatiotemporal gene expression control". Department of Biosystems Science and Engineering (D-BSSE), ETH–Zürich.

References