Composite

Part:BBa_K3163002:Design

Designed by: Burhanuddin Sabuwala, Neha Swaminathan, Shashank H S   Group: iGEM19_IIT-Madras   (2019-10-16)


eGFP with NOS terminator driven by a CaMV 35s promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1141
    Illegal BsaI.rc site found at 1428


Design Notes

As we intend to express this part in Fusarium solani, a fungus, a Kozak sequences had to be added in place of a bacterial RBS.


Source

The basic parts used to construct this composite part are the CamV35s promoter, eGFP and the NOS terminator. All three are present in the iGEM registry.

References