Composite

Part:BBa_K316031:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-20)


LytC cell wall binding domain with Pveg promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Pfu DNA polymerase was used to minimise PCR errors


Source

Genome PCR from B. subtilis genome (strain) with linker-TEV cleavable site-ComC synthesised and attached via a PME site within LytC gene

References