Part:BBa_K316031

LytC cell wall binding domain with Pveg promoter

We designed a protein that carries our signal peptide ComC (linear auto-inducing peptide from Streptococcus pneumoniae 2 component system) out of the cell where a protease has access to it. The protease can then proceed to cleave ComC off the protein, allowing quorum sensing via the the ComCDE system to take place.

One big problem we had to overcome is the cell wall that will obstruct the protease’s access to the protein carrying the signal peptide. The Detection module consists of a cell wall anchor, a signal peptide called ComC. A cleavable linker connects the two which has a recognition site for a specific protease we want to detect.

Please check our wiki peptide generator tool to see how this works [http://2010.igem.org/Team:Imperial_College_London/Software_Tool].

Figure I. Graphical representation of cell wall binding protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]