Coding
TEV

Part:BBa_K316012:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-23)


Design Notes

The part was produced by nucleotide synthesis by mwg – eurofins. Part BBa_K316000 contains a synthetic RBS was designed using Vogit lab RBS calculator[http://voigtlab.ucsf.edu/software/] to work with both 3'coding TEV protease and BBa_K316000.

Source

TEV protease is naturally found in Tobacco Etch Virus genome. This part contains the sequence codon optimized for expression in B.subtilis using mwg – eurofins [http://www.eurofinsdna.com/home.html] proprietary software.



TEV protease S219P autocatalysis resistant variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 32
  • 1000
    COMPATIBLE WITH RFC[1000]