Composite

Part:BBa_K3152005

Designed by: Xixi Song   Group: iGEM19_ASTWS-China   (2019-06-13)


mec operator-mCherry

This composite part is a report pathway. The mec operator is responsible for mCherry (BBa_J06504) expressing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

We contructed it successfully and took out two red colonies from the LB solid medium and digested by EcoRI and PstI restriction enzymes to further verify the correctness of the connection.

Figure 1 Photos of transformants of “pSB1C3-mec operator-mCherry” on LB agar broth.


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