Device

Part:BBa_K3151002:Design

Designed by: Jerresa Jabson   Group: iGEM19_Macquarie_Australia   (2019-09-12)


C-di-GMP Riboswitch Without a Terminator Stem Loop and eGFP Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 223
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205


Design Notes

In the eGFP site, ATG removed as it is in restriction site, extra TAA stop codon added


Source

Magnetospirillum magneticum

References