Measurement

Part:BBa_K315042:Design

Designed by: Curtiss Lane   Group: iGEM10_Davidson-MissouriW   (2010-07-22)

pLac-RBS-RFP-RBS-TetA (Deoptimized from bp 1-290)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1274
    Illegal NgoMIV site found at 1642
    Illegal NgoMIV site found at 1802
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This TetA gene has been deoptimized from base pairs 1 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was synthesized using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.

Source

This TetA gene has been deoptimized from base pairs 1 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was synthesized using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.

References