Coding

Part:BBa_K315040:Design

Designed by: Curtiss Lane   Group: iGEM10_Davidson-MissouriW   (2010-07-22)


TetA (Optimized from bp 1to 290)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 315
    Illegal NgoMIV site found at 683
    Illegal NgoMIV site found at 843
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

his TetA gene has been optimized from base pairs 1 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was synthesized using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.


Source

This TetA gene has been optimized from base pairs 1 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was synthesized using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.

References